Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in streptomyces lividans
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:518246
- Cornell Univ., Ithaca, NY (United States)
The major Thermomonospora fusca YX extracellular protease gene (tfpA) was cloned into Escherichia coli and Streptomyces lividans and was sequenced. The open reading frame encoded 375 residues, including a 31-residue potential signal sequence, an N-terminal prosequence containing 150 residues, and the 194-residue mature protease that belongs to the chymotrypsin family. The protease was secreted by S. lividans, but evidence suggested that it was bound to an extracellular protease inhibitor. An inhibitor-deficient mutant was selected to produce protease for purification. 30 refs., 4 figs.
- DOE Contract Number:
- FG02-84ER13233
- OSTI ID:
- 518246
- Journal Information:
- Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 11 Vol. 62; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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