Pancreatic islets of variable size - insulin secretion and glucose utilization

Journal Article · Mon Sep 16 04:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90597-1· OSTI ID:5180385

Colella, R M; Bonner-Weir, S; Braunstein, L P; Schwalke, M; Weir, G C

Glucose metabolism and insulin secretion were studied in isolated rat pacreatic islets of different sizes and the amount of tissue was quantitated by the measurement of DNA. It was found that larger islets (140-210 ng DNA/islet) utilized more glucose (based on the conversion of /sup 3/H-5-glucose to /sup 3/H/sub 2/O) per ng of DNA than islets containing less DNA (60-120 ng/islet). However, the insulin secreted per ng of DNA in response to a given glucose concentration was the same in islets of all sizes. Also, the islet insulin and glucagon content when expressed in terms of DNA did not depend upon islet size. Thus, although glucose utilization rates expressed as a function of islet DNA content were greater in larger islets, no such relationship was found for glucose-induced insulin release or insulin and glucagon content. 17 reference, 1 figure, 3 tables.

• Research Organization: Medical College of Virginia, Richmond
• OSTI ID: 5180385
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:11; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
DNA
ENDOCRINE GLANDS
GLANDS
GLUCOSE
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
RATS
RODENTS
SACCHARIDES
SECRETION
SIZE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES