Sympathetic denervation does not alter the density or properties of alpha-1 adrenergic receptors in rat vas deferens

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · J. Pharmacol. Exp. Ther.; (United States)

OSTI ID:5180143

Abel, P W; Johnson, R D; Martin, T J; Minneman, K P

Alpha-1 adrenergic receptors in surgically denervated rat vas deferens were studied using radioligand binding assays of (/sup 125/I) BE 2254 ((/sup 125/I)BE) and contraction measurements. Scatchard analysis of saturation isotherms of specific (/sup 125/I)BE binding showed no change in the affinity or density of binding sites 4, 7 or 14 days after denervation of rat vas deferens. The potency of norepinephrine in inhibiting specific (/sup 125/I)BE binding was also unchanged 7 days after denervation of vas deferens. The potency of phenylephrine in causing contractions in vitro did not change 4, 7 or 14 days after denervation of vas deferens; however, there was a significant increase in the maximum contractile response to phenylephrine at all time points. After partial inactivation of alpha-1 adrenergic receptors in vitro with phenoxybenzamine, there was an equivalent reduction in the number of (/sup 125/I)BE binding sites in the control and 14-day denervated vas deferens. The equilibrium dissociation constants calculated from contractile measurements for norepinephrine were the same in the control and denervated tissues. However, there was a 2.2-fold increase in contractile sensitivity to norepinephrine 14 days after denervation and a 3.6-fold increase in contractile sensitivity to methacholine 7 days after denervation.

• Research Organization: Emory Univ. School of Medicine, Atlanta, GA
• OSTI ID: 5180143
• Journal Information: J. Pharmacol. Exp. Ther.; (United States), Journal Name: J. Pharmacol. Exp. Ther.; (United States) Vol. 3; ISSN JPETA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
AFFINITY
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL BONDS
CHOLINE
CONTRACTION
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GONADS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTHERMS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIPOTROPIC FACTORS
MALE GENITALS
MAMMALS
MUSCLES
NERVES
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SENSITIVITY
STEROID HORMONES
SYMPATHOMIMETICS
TESTES
TRACER TECHNIQUES
VERTEBRATES