Determinants of action of phospholipases A2 on the envelope phospholipids of Escherichia coli
Thesis/Dissertation
·
OSTI ID:5179838
The rabbit neutrophil phospholipase A2 (PLA2) degrades the phospholipids of E. coli killed by a bactericidal protein purified from the rabbit neutrophil. Of nine other PLA2 examined four were inactive, four produced low levels of hydrolysis, and one, the basic isozyme of the Agkistrodon halys blomhoffii venom, was very active. Because of the highly cationic properties of the basic Agkistrodon PLA2(pl = 10.2) the author examined the role of net positive charge in the ability of the PLA2 to act on the phospholipids of E. coli. Lysines in the basic venom PLA2 were chemically modified using two procedures, reductive methylation and carbamylation. Modification of up to 4 Lys/molecule did not alter catalytic activity as measured by using artificial substrates. In contrast, the ability of the basic PLA2 to act on E. coli exposed to the bactericidal protein was reduced by 70% when 1 Lys/molecule had been modified. The primary sequence of the basic PLA2 was determined and the specific radioactivity of individual carbamylated lysines was measured when 1 mol of (14C)cyanate/mol of protein had been incorporated. X-ray structure analysis by others of 3 PLA2 and secondary structure reductions of 30 PLA2, including the basic PLA2, indicate that the first 13 residues of the basic PLA2 are arranged in an alpha helical conformation.
- Research Organization:
- New York Univ., NY (USA)
- OSTI ID:
- 5179838
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
CONFIGURATION
CYANATES
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
ESTERS
HELICAL CONFIGURATION
HYDROLASES
IONIZING RADIATIONS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIPASE
LIPIDS
LYSINE
MAMMALS
MATERIALS
MICROORGANISMS
NEUTROPHILS
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RABBITS
RADIATIONS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
VERTEBRATES
X RADIATION
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
CONFIGURATION
CYANATES
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
ESTERS
HELICAL CONFIGURATION
HYDROLASES
IONIZING RADIATIONS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIPASE
LIPIDS
LYSINE
MAMMALS
MATERIALS
MICROORGANISMS
NEUTROPHILS
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RABBITS
RADIATIONS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
VERTEBRATES
X RADIATION