Effect of solvent and extraction methods on the bacterial mutagenicity of sidestream cigarette smoke

Technical Report · Wed Dec 31 23:00:00 EST 1986

OSTI ID:5178159

Morin, R S; Tulis, J J; Claxton, L D

The mutagenic activity of sidestream cigarette-smoke particles was estimated by testing sidestream cigarette-smoke particles that had been collected under controlled burning conditions in the laboratory. Two different extraction methods (Soxhlet and ultrasonic agitation) and 3 different solvents (dichloromethane, methanol, and acetone) were compared for their efficiencies in the extraction of compounds from sidestream cigarette-smoke particles that are mutagenic in the Ames test. The mutagenic activity of the sidestream smoke particles was estimated to be 15,000-20,000 revertants per cigarette in TA98 with metabolic activation and 12,000-17,000 revertants per cigarette in TA100 without metabolic activation.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 5178159
• Report Number(s): PB-88-182381/XAB; EPA-600/J-87/256
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
ACETONE
AEROSOLS
AIR POLLUTION
BACTERIA
BIOLOGY
COLLOIDS
DISPERSIONS
EXTRACTION
GENETICS
KETONES
LABORATORIES
METABOLISM
MICROORGANISMS
MUTAGENESIS
MUTAGENS
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
POLLUTION
RESIDUES
SALMONELLA
SEPARATION PROCESSES
SMOKES
SOLS
SOLVENT EXTRACTION
TOBACCO SMOKES
TOXICITY