Prostaglandin F/sub 2. cap alpha. activates phosphoinositide hydrolysis in rat aorta

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5177593

The authors have previously demonstrated that norepinephrine (NE) and serotonin (5HT) activate a phosphoinositide-(PI) specific phospholipase C in rat aorta by interaction with ..cap alpha../sub 1/-adrenergic receptors and 5HT/sub 2/ receptor, respectively. They have subsequently noted that angiotensin II and vasopressin as well activate PI hydrolysis in the tissue. The most active agent they have thus far investigated is prostaglandin F/sub 2..cap alpha../ (PGF/sub 2..cap alpha../). Rat aortic rings were pre-labelled with (/sup 3/H)-inositol and then, in the presence of 10 mM LiCl, exposed to various doses of PGF/sub 2..cap alpha../. (/sup 3/H)-inositol monophosphate was the quantified by anion-exchange chromatography. After a 60 min incubation, PGF/sub 2..cap alpha../ caused a 10-15 fold increase over basal at maximal concentrations (0.1-1.0 mM). An EC/sub 50/ for PI hydrolysis was between 0.1-1.0 ..mu..M. PGF/sub 2..cap alpha../ caused maximal aortic contraction at 10 ..mu..M. PGF/sub 2..cap alpha../-induced PI hydrolysis, was inhibited by phorbol esters. These results suggest that PGF/sub 2..cap alpha../, similar to 5HT, NE, vasopressin and angiotensin II, causes vasoconstriction by activation of PI hydrolysis.

• Research Organization: Naval Medical Research Institute, Bethesda, MD
• OSTI ID: 5177593
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AORTA
VASOCONSTRICTION
INOSITOL
HYDROLYSIS
PROSTAGLANDINS
BIOLOGICAL EFFECTS
ION EXCHANGE CHROMATOGRAPHY
LITHIUM CHLORIDES
MUSCLES
PHOSPHATES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
ARTERIES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DECOMPOSITION
HALIDES
HALOGEN COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LITHIUM COMPOUNDS
LITHIUM HALIDES
LYSIS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
VERTEBRATES
550901* - Pathology- Tracer Techniques