Fluorometric method of quantitative cell mutagenesis

Patent · 12 December 1980

OSTI ID:5177161

Dolbeare, F A

A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.

• DOE Contract Number: W-7405-ENG-48
• Assignee: Dept. of Energy
• Patent Number(s): None
• OSTI ID: 5177161
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACCURACY
BANDING TECHNIQUES
BIOASSAY
CELL CULTURES
CHEMICAL ANALYSIS
FLUORESCENCE
HISTOLOGICAL TECHNIQUES
HISTOLOGY
LUMINESCENCE
MEASURING METHODS
MUTAGENESIS
QUANTITATIVE CHEMICAL ANALYSIS
STAINS