Structure of the gene encoding human complement component
- Univ. of Alabama, Birmingham (United States)
A genomic cosmid clone, S22A, was used to elucidate the structure of the human C2 gene. Eighteen exons spanning 18 kbp of DNA were mapped by Southern blotting, PCR, and nucleotide sequencing. They contain all but the first 115 bp of the 5{prime}-UTR of the C2 cDNA, C2HL5-3. Introns vary in length from 83 bp to 4.4 kbp. All intron/exon boundaries follow of AG/GT consensus rule for splicing. The three SCRs of C2b are encoded by exons 2, 3, and 4, respectively. The von Willebrand factor-like domain of C2a is encoded by 6 exons. The serine proteinase domain of C2a is encoded by 8 exons with the putative catalytic center and substrate binding residues encoded by separate exons. Exons 5 and 15 appear to be unique to the C2 and factor B genes. Overall, the structures of the genes for C2 and factor B are highly homologous differing only in the size of introns.
- OSTI ID:
- 5176307
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
The organization of the human complement factor I gene (IF): A member of the serine protease gene family
The structural organization of the human skeletal muscle ryanodine receptor (RYR1) gene