Enzymic and physicochemical characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from diploid and tetraploid cultivars of perennial ryegrass

Journal Article · Tue Sep 01 04:00:00 EDT 1981 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(81)90228-9· OSTI ID:5175149

Rejda, J M; Johal, S; Chollet, R

Homogeneous preparations of ribulose 1,5-bisphosphate carboxylase/oxygenase were isolated from several diploid and tetraploid cultivars of perennial ryegrass by three different purification protocols. The apparent K/sub m/ values for substrate CO/sub 2/ were essentially identical for the fully CO/sub 2//Mg/sup 2 +/-activated diploid and tetraploid enzymes, as were the kinetics for deactivation and activation of the CO/sub 2//Mg/sup 2 +/-activated and -depleted carboxylases, respectively. Similarly, virtually indistinguishable electrophoretic properties were observed for both the native and dissociated diploid and tetraploid ryegrass proteins, including native and subunit molecular weights and the isoelectric points of the native proteins and the large and small subunit component polypeptides. The quantity of carboxylase protein or total soluble leaf protein did not differ significantly between the diploid and tetraploid cultivars. Contrary to a previous report, these results indicate that increased ploidy level has had essentially no effect on the quantity or enzymic and physicochemical properties of ribulosebisphosphate carboxylase/oxygenase in perennial ryegrass.

• Research Organization: Univ. of Nebraska, Lincoln
• DOE Contract Number: AC02-81ER10902
• OSTI ID: 5175149
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Journal Issue: 2 Vol. 210:2; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGY
CARBOHYDRATES
CARBON
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLASE
CEREALS
CHALCOGENIDES
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GENETICS
GRAMINEAE
GRASS
IONS
KETONES
KINETICS
LEAVES
LIGASES
MAGNESIUM IONS
METABOLISM
MOLECULAR BIOLOGY
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PENTOSES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYSIOLOGY
PLANTS
PLOIDY
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
REACTION KINETICS
RIBULOSE
RYE
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS