Dietary zinc affects the amount of molecular distribution of zinc in endogenous secretions
- Dept. of Agriculture, Grand Forks, ND (United States)
Long-Evans rats were used to examine the effect of dietary Zn on Zn secreted into endogenous secretions. Animals were fed diets containing 8, 20, or 60 mg Zn per kg diet for six weeks. Rats were then anesthetized, opened at midline and polyethylene cannulas were inserted into the common duct or bile duct for collection of bile/pancreatic fluid or pure bile. Additionally, some rats were injected with 65-Zn 48h prior to cannulation. Secretions were chromatographed on G-75 Sephadex equilibrated with 0.1 M Tris, pH 8.0. Fractions were collected and analyzed for Zn and 65-Zn. More Zn and 65-Zn were found in the pancreatic/biliary secretions than in pure bile. The amount of 65-Zn in bile/pancreatic secretions varied with diet. Zinc and 65-Zn from pancreatic/biliary secretions were found in low and high molecular weight fractions with the greatest amount contained in the high molecular weight fraction. The molecular weight of ligands binding 65-Zn was not affected by diet; however, less 65-Zn was associated with the low molecular weight fraction in rats fed 8 ppm than in animals fed 60 ppm Zn. Non-radioactive bile/pancreatic fluid was incubated with 65-Zn and also chromatographed. Secretions labeled in this way contained most 65-Zn in the low molecular weight fraction. These results suggest that, within a physiological range, the secretion of endogenous Zn mirrors Zn intake. A low molecular weight Zn ligand in bile/pancreatic fluid apparently is most responsive to changes in dietary Zn. These data also suggest that 65-Zn added exogenously does not completely exchange with endogenously secreted Zn.
- OSTI ID:
- 5174724
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biliary excretion of cadmium by rats: effects of zinc, cadmium, and selenium pretreatments
Comparison of soy-protein and egg albumin on endogenously secreted zinc
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BILE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIET
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METABOLISM
METALS
MOLECULAR WEIGHT
NUCLEI
ORGANS
PANCREAS
RADIOISOTOPES
RATS
RODENTS
SECRETION
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
ZINC
ZINC 65
ZINC ISOTOPES