The interaction between dietary Fe, Cu and stress in Cu-67 retention and serum ceruloplasmin (Cp) activity in rats
- Univ. of Missouri, Columbia (United States)
The objectives of the study were to determine the effects of dietary Fe and stress on Cu-67 retention and serum Cp activity in the rat. A 2 {times} 2 {times} 2 factorial arrangement of treatments was utilized. Male Sprague Dawley weanling rats were fed AIN-76 diets ad lib containing 0.8 ppm Cu (CuD) or 5.7 ppm Cu (CuA) with 22.5 ppm Fe (FeA) or 280 ppm Fe (FeE). After 19 days, one-half of the animals of each treatment were stressed by an intramuscular injection of 0.1 ml turpentine/100 gm body weight. Forty-eight hours later, animals were gavaged with Cu-67 and counted over a 7 day period in a whole body high resolution gamma counter. Cu-67 retention was 20% higher in CuD rats compared to CuA rats. There were no significant effects caused by Fe or stress or the interaction between these variables on Cu-67 retention. In rats fed FeE-CuA diets, serum Cp activity was significantly depressed compared to rats fed FeA-CuA diets. These reductions in the acute phase protein Cp, were 85% and 70% in nonstressed and stressed rats, respectively. The results of this study suggest that the negative interaction effects of excess Fe on Cu utilization does not occur at the site of Cu absorption, but within the body and specifically in the liver.
- OSTI ID:
- 5172413
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CERULOPLASMIN
BIOSYNTHESIS
COPPER
METABOLISM
IRON
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
COPPER 67
DIET
LIVER
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
COMPLEXES
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
GLOBULINS
GLOBULINS-ALPHA
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALLOPROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques