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Acute effects of ethanol on hepatic uptake and distribution of narcotics in the isolated perfused rabbit liver

Journal Article · · Hepatology (N.Y.); (United States)
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This study was performed as an initial step in systematically defining the hepatic interactions between ethanol and opioids using a controlled in vitro system. The acute effects of ethanol on the initial uptake and distribution of long- and short-acting narcotics were studied using isolated rabbit liver perfused with rabbit blood without or with ethanol. A pulse injection of 1.5 mg of 14C-labeled narcotic (methadone, 1-alpha-acetylmethadol (LAAM), morphine, or meperidine) was made into the portal vein cannula followed by perfusion for 2 min. Radioactivity was determined in liver homogenates and subcellular fractions; methadone and its metabolites were measured by thin-layer chromatography with zonal scanning in each fraction. Ethanol preperfusion and concomitant ethanol perfusion did not effect hepatic uptake of methadone, LAAM, morphine, or meperidine. Although subcellular localization of morphine and meperidine differed from that of methadone and LAAM, perfusion with ethanol did not alter the acute hepatic uptake and distribution of any of the narcotics. These findings suggest that acute exposure to ethanol does not alter the acute hepatic disposition of narcotics.
Research Organization:
Rockefeller University, Department of Nuclear Medicine, New York, New York
OSTI ID:
5172161
Journal Information:
Hepatology (N.Y.); (United States), Journal Name: Hepatology (N.Y.); (United States) Vol. 1:5; ISSN HEPAD
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALOIDS
ANALGESICS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DRUGS
ETHANOL
GLANDS
HYDROXY COMPOUNDS
IN VITRO
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METHADONE HYDROCHLORIDE
MORPHINE
NARCOTICS
OPIUM
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
RABBITS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
UPTAKE
VERTEBRATES