Sol-gel glasses as new waveguides for optical immunosensors
- Centre d`Etudes du Bouchet, Vert-le-Petit (France)
- Ecole Polytechnique, Palaiseau (France)
- Inst. d`Optique Theorique et Appliquee, Orsay (France)
In order to develop an optical immunosensor based on Surface Plasmon Resonance (SPR) and guided waves propagation, the sol-gel process has been used to prepare thin films of amorphous silica, deposited by spin coating on a gold-coated glass slide, and possessing chemically active functional groups (SH, NH{sub 2} ... ). After activation of the Sol-gel film in aqueous buffers by a bifunctional coupling agent, biological molecules such as antibodies could be covalently bonded on or inside the sol-gel film. Therefore, the behavior of the functionalized silica thin films in aqueous solutions has been analyzed by SPR and guided waves propagation. The matrix hydrophilicity has been studied with different mixtures of functionalized silicon precursors forming organic-inorganic hybrid films. Immunoassays outline the possibility of obtaining covalent binding of antibodies to the sol-gel matrix with optimal stability and biological activity.
- OSTI ID:
- 51678
- Report Number(s):
- CONF-940411-; ISBN 1-55899-246-4; TRN: IM9524%%143
- Resource Relation:
- Conference: Spring meeting of the Materials Research Society (MRS), San Francisco, CA (United States), 4-8 Apr 1994; Other Information: PBD: 1994; Related Information: Is Part Of Better ceramics through chemistry VI. Materials Research Society symposium proceedings Volume 346; Cheetham, A.K. [ed.] [Univ. of California, Santa Barbara, CA (United States)]; Brinker, C.J. [ed.] [Sandia National Labs., Albuquerque, NM (United States)]; Mecartney, M.L. [ed.] [Univ of California, Irvine, CA (United States)]; Sanchez, C. [ed.] [Univ. Pierre et Marie Curie, Paris (France)]; PB: 1069 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
NiO-silica based nanostructured materials obtained by microemulsion assisted sol-gel procedure
Optical sensors based on sol-gel derived, laminate planar waveguide structures