One-carbon metabolism in anaerobes: Organic acid and methane production: Final technical report for period June 1, 1985-May 31,1987
Objectives of this project dealt with understanding one- and multicarbon metabolism in acetogenic and methanogenic bacteria. The biochemistry of acetate catabolism in Methanosarcina barkeri in relation to their major enzyme activities, co-factors and intermediates were studied. The physiological function of CO-dehydrogenase was investigated and found to be involved in hydrogen production when CO was used as substrate. Hydrogen metabolism from acetate by Methanosarcina barkeri was investigated through isotope tracer studies. Results showed that hydrogen production appears to be linked to several intracellular redox processes which follow the cleavage of acetate. The metabolic pathway in Butyribacterium methylotrophicum for multicarbon metabolism was elucidated. The key enzyme, ferredoxin-NAD reductase, was found to be regulated by NADH. The one carbon and multicarbon metabolic pathways in Butyribacterium methylotrophicum were constituted. Hydrogen was produced through multicarbon metabolism and was consumed by one carbon metabolism through the reduction of CO/sub 2/. Results revealed why high efficiency of carbon and energy conservation was observed in acetogens. The catabolism of formate to acetate by this microorganism was analyzed by fermentation time course and /sup 13/C nuclear magnetic resonance studies. Results indicate a bifurcated single-carbon catabolic pathway in which CO/sub 2/ is the sole single-carbon compound that directly supplies the carbonyl and methyl group synthesis pathways leading to the formation of acetyl coenzyme A, the primary reduced product. 1 fig.
- Research Organization:
- Michigan State Univ., East Lansing (USA). Dept. of Biochemistry
- DOE Contract Number:
- FG02-85ER13376
- OSTI ID:
- 5166973
- Report Number(s):
- DOE/ER/13376-2; ON: DE88008137
- Country of Publication:
- United States
- Language:
- English
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One carbon metabolism in anaerobic bacteria: Regulation of carbon and electron flow during organic acid production
One carbon metabolism in anaerobic bacteria: organic acid and methane production. Final technical report
Related Subjects
08 HYDROGEN
59 BASIC BIOLOGICAL SCIENCES
ACETATES
CATABOLISM
ANAEROBIC DIGESTION
FORMATES
HYDROGEN
PRODUCTION
METHANE
BIOSYNTHESIS
METHANOGENIC BACTERIA
BIOLOGICAL PATHWAYS
NUCLEOTIDE DEHYDROGENASES
BIOCHEMICAL REACTION KINETICS
OXIDOREDUCTASES
IMMUNOASSAY
CARBON 13
CARBON DIOXIDE
CARBON MONOXIDE
CHEMICAL REACTION YIELD
COENZYMES
DNA-CLONING
ELECTRON TRANSFER
ENZYME ACTIVITY
FERREDOXIN
METABOLISM
MONOCLONAL ANTIBODIES
NUCLEAR MAGNETIC RESONANCE
PROGRESS REPORT
REACTION INTERMEDIATES
TRACER TECHNIQUES
ALKANES
ANTIBODIES
BACTERIA
BIOCONVERSION
CARBON COMPOUNDS
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CLONING
DIGESTION
DOCUMENT TYPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MANAGEMENT
METALLOPROTEINS
MICROORGANISMS
NONMETALS
NUCLEI
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROCESSING
PROTEINS
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
SYNTHESIS
WASTE MANAGEMENT
WASTE PROCESSING
YIELDS
090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)
080106 - Hydrogen- Production- Biosynthesis & Photochemical Processes
550200 - Biochemistry