One-carbon metabolism in anaerobes: Organic acid and methane production: Final technical report for period June 1, 1985-May 31,1987

Technical Report · Thu Oct 01 04:00:00 EDT 1987

OSTI ID:5166973

Zeikus, J G

Objectives of this project dealt with understanding one- and multicarbon metabolism in acetogenic and methanogenic bacteria. The biochemistry of acetate catabolism in Methanosarcina barkeri in relation to their major enzyme activities, co-factors and intermediates were studied. The physiological function of CO-dehydrogenase was investigated and found to be involved in hydrogen production when CO was used as substrate. Hydrogen metabolism from acetate by Methanosarcina barkeri was investigated through isotope tracer studies. Results showed that hydrogen production appears to be linked to several intracellular redox processes which follow the cleavage of acetate. The metabolic pathway in Butyribacterium methylotrophicum for multicarbon metabolism was elucidated. The key enzyme, ferredoxin-NAD reductase, was found to be regulated by NADH. The one carbon and multicarbon metabolic pathways in Butyribacterium methylotrophicum were constituted. Hydrogen was produced through multicarbon metabolism and was consumed by one carbon metabolism through the reduction of CO/sub 2/. Results revealed why high efficiency of carbon and energy conservation was observed in acetogens. The catabolism of formate to acetate by this microorganism was analyzed by fermentation time course and /sup 13/C nuclear magnetic resonance studies. Results indicate a bifurcated single-carbon catabolic pathway in which CO/sub 2/ is the sole single-carbon compound that directly supplies the carbonyl and methyl group synthesis pathways leading to the formation of acetyl coenzyme A, the primary reduced product. 1 fig.

• Research Organization: Michigan State Univ., East Lansing (USA). Dept. of Biochemistry
• DOE Contract Number: FG02-85ER13376
• OSTI ID: 5166973
• Report Number(s): DOE/ER/13376-2; ON: DE88008137
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106 -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
09 BIOMASS FUELS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
ANAEROBIC DIGESTION
ANTIBODIES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 13
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON MONOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CATABOLISM
CHALCOGENIDES
CHEMICAL REACTION YIELD
CLONING
COENZYMES
DIGESTION
DNA-CLONING
DOCUMENT TYPES
ELECTRON TRANSFER
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FERREDOXIN
FORMATES
HYDROCARBONS
HYDROGEN
IMMUNOASSAY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MANAGEMENT
METABOLISM
METALLOPROTEINS
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
MONOCLONAL ANTIBODIES
NONMETALS
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDE DEHYDROGENASES
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROCESSING
PRODUCTION
PROGRESS REPORT
PROTEINS
REACTION INTERMEDIATES
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
WASTE MANAGEMENT
WASTE PROCESSING
YIELDS