Cell-mediated mutagenesis and cell transformation by chemical carcinogens
Conference
·
OSTI ID:5166193
Results are reported from studies that showed that mutagenesis of mammalian cells can be achieved by carcinogenic polycyclic hydrocarbons, nitrosamines, and aflatoxins when tested in the presence of fibroblasts and hepatocytes which are able to metabolize these carcinogens. Further, we have found that there is a relationship between the degree of mutant induction and the degree of carcinogenicity of the different chemicals tested. By simultaneously measuring the frequency of cell transformation and the frequency of mutation at one locus (ouabain resistance) in the same cell system, it was possible to estimate the genetic target site for cell transformation. The results indicated that the target site for transformation is approximately 20 times larger than that determined for ouabain resistance. The results suggest that cell transformation may be due to a mutational event and the mutation can occur in one out of a small number of the same or different genes, and that the cell-mediated mutagenesis approach may be a valuable means of detecting tissue-specific carcinogens.
- Research Organization:
- Oak Ridge National Lab., Tenn. (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5166193
- Report Number(s):
- CONF-7706119-1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
AMINES
ANIMAL CELLS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENESIS
CARCINOGENS
CARDIOTONICS
CELL CULTURES
DRUGS
GENETIC CONTROL
GLYCOSIDES
HYDROCARBONS
KINETICS
METABOLISM
MUTAGENESIS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PATHOGENESIS
PEST CONTROL
POLYCYCLIC AROMATIC HYDROCARBONS
REACTION KINETICS
SENSITIVITY
STEROIDS
STROPHANTIN
TRANSCRIPTION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
AMINES
ANIMAL CELLS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENESIS
CARCINOGENS
CARDIOTONICS
CELL CULTURES
DRUGS
GENETIC CONTROL
GLYCOSIDES
HYDROCARBONS
KINETICS
METABOLISM
MUTAGENESIS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PATHOGENESIS
PEST CONTROL
POLYCYCLIC AROMATIC HYDROCARBONS
REACTION KINETICS
SENSITIVITY
STEROIDS
STROPHANTIN
TRANSCRIPTION