Utilization of carbohydrates by thermomonospora sp. grown on glucose, cellobiose, and cellulose

Journal Article · Mon Jun 01 04:00:00 EDT 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230612· OSTI ID:5165538

Moreira, A R; Phillips, J A; Humphrey, A E

Thermomonospora sp. was grown on glucose, cellobiose, and cellulose in order to study its growth characteristics with different carbohydrate substrates. It was observed that the nitrogen and protein contents of the cells are essentially constant during the fermentation and independent of the carbon source when glucose or cellobiose are utilized. Under oxygen starvation conditions it was shown that unidentifiable organic compound(s) accumulate(s) in the culture broth. Culture fluorescence was shown to be an excellent variable for monitoring and control of the fermentation process. This microorganism showed a preference for crystalline cellulose (Avicel) as substrate although it grows readily on a more amorphous cellulose (Solka Floc). The production of extracellular protein is shown to be growth related. Data were obtained confirming the decrease in the number of active adsorption sites as the cause for the decrease in the cellulose digestion rate. 17 refs.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5165538
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CELL CULTURES
CELLOBIOSE
CELLULOSE
DISACCHARIDES
ELEMENTS
FERMENTATION
FLUORESCENCE
GLUCOSE
GROWTH
HEXOSES
LUMINESCENCE
MICROORGANISMS
MONOSACCHARIDES
NITROGEN
NONMETALS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN
PLANT GROWTH
POLYSACCHARIDES
PROTEINS
SACCHARIDES