Crude oil effects on microsomal mixed-function oxidase system components in the striped mullet (Mugil cephalus)

Journal Article · Sat Oct 15 04:00:00 EDT 1977 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(77)90107-2· OSTI ID:5165443

Yarbrough, J D; Chambers, J E

The effects of exposure to two types of crude oil on microsomal mixed-function oxidase system components in livers of juvenile striped mullet (Mugil cephalus) were investigated. Mullet were exposed for 4 days to emulsified Empire Mix or Saudi Arabian crude oils at an initial concentration of 75 ppM and an average of 1 ppM in the water column. Liver size was increased by about 50 percent following exposure to both oils. Since neither total hepatic protein nor microsomal protein increased as rapidly as did liver size, the concentrations of both were reduced following oil exposures. The proportion of microsomal protein to total hepatic protein or wet weight was not altered following crude oil exposure. Both cytochromes P-450 and b/sub 5/ were induced following oil treatment. NADPH-dependent enzymes assayed with cytochrome c and dichlorophenolindophenol as substrates showed increases in activity after exposure to Empire Mix crude oil but only the latter enzyme activity was increased on a microsomal protein basis following Saudi Arabian crude oil treatment. Activities of NADH cytochrome c and NADH cytochrome b/sub 5/ reductases appeared to vary with the protein level. However, since liver size was increased, oil-treated mullet had more of all parameters measured than did control mullet. Although the acute toxicity of Saudi Arabian crude oil to mullet is greater than that of Empire Mix crude oil, Empire Mix crude oil had greater inductive effects on microsomal oxidase components.

• Research Organization: Mississippi State Univ., State College
• OSTI ID: 5165443
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 21:8; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020900 -- Petroleum-- Environmental Aspects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BODY
CATALYSIS
CELL CONSTITUENTS
CYTOCHROME OXIDASE
CYTOCHROMES
DIGESTIVE SYSTEM
ENERGY SOURCES
ENZYMES
FISHES
FOSSIL FUELS
FUELS
GLANDS
LIVER
METABOLISM
MICROSOMES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDASES
OXIDOREDUCTASES
PETROLEUM
PIGMENTS
PROTEINS
SIZE
TOXICITY
VERTEBRATES