Transfer of 45Ca and 36Cl at the blood-nerve barrier of the sciatic nerve in rats fed low or high calcium diets

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Journal of Neuroscience Research; (United States)

DOI:https://doi.org/10.1002/jnr.490280413· OSTI ID:5163112

Wadhwani, K C; Murphy, V A; Rapoport, S I ^[1]

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1. Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD (USA)

Unidirectional fluxes of 45Ca, 36Cl, and of (3H)mannitol from blood into the sciatic nerve and cerebral cortex were determined from 5- and 15-min uptakes of these tracers after an intravenous (i.v.) bolus injection in awake rats. Rats were fed diets for 8 wk, that had either a low (0.01% wt/wt), normal (0.67%), or high (3%) Ca content. Plasma (Ca) was 32% less and 11% more in rats fed low (LOCA) and high Ca diets (HICA), respectively, than in rats fed a normal Ca diet (CONT). The mean permeability-surface area product (PA) of 45Ca at the blood-nerve barrier was about eightfold higher than at the blood-brain barrier in the same animals and did not differ significantly between groups (greater than 0.05). Mean PA ratios of 45Ca/36Cl for the blood-nerve and blood-brain barriers in CONT rats, 0.52 {plus minus} 0.04 and 0.40 {plus minus} 0.02, respectively, were not significantly different from corresponding ratios in LOCA and HICA groups, and corresponded to the aqueous limiting diffusion ratio (0.45). The authors results show no evidence for concentration-dependent transport of Ca over a plasma (Ca) range of 0.8-1.4 mmol/liter at the blood-nerve barrier of the rat peripheral nerve, and suggest that Ca and Cl exchange slowly between nerve and blood via paracellular pathways.

• OSTI ID: 5163112
• Journal Information: Journal of Neuroscience Research; (United States), Vol. 28:4; ISSN 0360-4012
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD-BRAIN BARRIER
PERMEABILITY
CALCIUM
MEMBRANE TRANSPORT
CHLORIDES
BIOLOGICAL PATHWAYS
CALCIUM 45
CHLORINE 36
HOMEOSTASIS
ISOTOPE RATIO
RATS
SCIATIC NERVE
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METALS
NERVES
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
RADIOISOTOPES
RODENTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques