Multinuclear NMR studies of hemoproteins and their model compounds
Thesis/Dissertation
·
OSTI ID:5160645
Nuclear magnetic resonance (NMR) in both solution and solid state has been used to study the active site structure of various hemoproteins, and the nature of the iron-oxygen bond in oxyhemoglobin. The first iron-57 NMR spectra of a metalloprotein, carbonmonoxymyoglobin, has been obtained, yielding the isotropic chemical shift, the anisotropy of the chemical shielding tensor and the rotational correlation time of the protein. The oxygen-17 NMR signals from CO ligands bound to oxygen-transport hemoproteins are much narrower than expected, and the lineshape is non-Lorentzian. The results indicate that the unusual linewidths and lineshapes originate from the multiexponential nature of quadrupolar relaxation outside of the extreme narrowing limit, permitting determinations of the oxygen-17 nuclear quadrupole coupling constants and the rotational correlation time of the proteins.
- Research Organization:
- Illinois Univ., Urbana, IL (USA)
- OSTI ID:
- 5160645
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
CHEMICAL SHIFT
EVEN-ODD NUCLEI
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METALLOPROTEINS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN 17
OXYGEN ISOTOPES
PIGMENTS
PORPHYRINS
PROTEINS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
CHEMICAL SHIFT
EVEN-ODD NUCLEI
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METALLOPROTEINS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN 17
OXYGEN ISOTOPES
PIGMENTS
PORPHYRINS
PROTEINS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES