Metabolism of ( sup 14 C)cholesterol to C-20 isomeric ( sup 14 C)pregn-5-ene-3,20-diols in the tobacco hornworm, Manduca sexta

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Steroids; (USA)

DOI:https://doi.org/10.1016/0039-128X(89)90023-8· OSTI ID:5159693

Lozano, R; Thompson, M J; Svoboda, J A; Lusby, W R; Wilzer, Jr, K R ^[1]

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1. Insect and Nematode Hormone Laboratory, Beltsville, MD (USA)

After injection into male and female fifth-instar larvae of Manduca sexta, ({sup 14}C)cholesterol was converted to C21 steroids, ({sup 14}C)pregn-5-ene-3 beta,20-diols. These metabolites were isolated from 8-day-old pupae and were identified by TLC, HPLC, and GC-MS as the C-20 isomers of pregnene-3 beta,20-diol. They also were isolated from male and female meconium fluid (of 16-day-old pupae) following injection of ({sup 14}C)cholesterol into 14-day-old pupae.

• OSTI ID: 5159693
• Journal Information: Steroids; (USA), Vol. 53:3-5; ISSN 0039-128X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLESTEROL
METABOLISM
CARBON 14 COMPOUNDS
GAS CHROMATOGRAPHY
LARVAE
LEPIDOPTERA
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTRA
MASS SPECTROSCOPY
METABOLITES
SPECTROPHOTOMETRY
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
ANIMALS
ARTHROPODS
CHROMATOGRAPHY
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
STEROIDS
STEROLS
550501* - Metabolism- Tracer Techniques