Metabolism of ( sup 14 C)cholesterol to C-20 isomeric ( sup 14 C)pregn-5-ene-3,20-diols in the tobacco hornworm, Manduca sexta

Journal Article · Tue Feb 28 23:00:00 EST 1989 · Steroids; (USA)

DOI:https://doi.org/10.1016/0039-128X(89)90023-8· OSTI ID:5159693

Lozano, R; Thompson, M J; Svoboda, J A; Lusby, W R; Wilzer, Jr, K R ^[1]

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1. Insect and Nematode Hormone Laboratory, Beltsville, MD (USA)

After injection into male and female fifth-instar larvae of Manduca sexta, ({sup 14}C)cholesterol was converted to C21 steroids, ({sup 14}C)pregn-5-ene-3 beta,20-diols. These metabolites were isolated from 8-day-old pupae and were identified by TLC, HPLC, and GC-MS as the C-20 isomers of pregnene-3 beta,20-diol. They also were isolated from male and female meconium fluid (of 16-day-old pupae) following injection of ({sup 14}C)cholesterol into 14-day-old pupae.

• OSTI ID: 5159693
• Journal Information: Steroids; (USA), Journal Name: Steroids; (USA) Vol. 53:3-5; ISSN 0039-128X; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
CARBON 14 COMPOUNDS
CHOLESTEROL
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LARVAE
LEPIDOPTERA
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTRA
MASS SPECTROSCOPY
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTRA
SPECTROPHOTOMETRY
SPECTROSCOPY
STEROIDS
STEROLS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES