Protein kinase C and. alpha. 2-adrenoceptor-mediated inhibition of noradrenaline release from the rat tail artery

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Journal of Cardiovascular Pharmacology; (United States)

OSTI ID:5158526

Bucher, B; Neuburger, J; Illes, P ^[1]

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1. Laboratoire de Pharmacologie Cellulaire et Moleculaire, C.N.R.S. URA 600, Universite Louis Pasteur Strasbourg, Illkirch (France)

In isolated rat tail arteries preincubated with (3H)noradrenaline, electrical field stimulation evoked the overflow of tritium. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activating phorbol ester, time-dependently increased the overflow at 1 mumol/L but not at 0.1 mumol/L. In contrast, the overflow was not altered by phorbol 13-acetate (PA, 1 mumol/L), which does not influence the activity of PKC. Polymyxin B (70 mumol/L), an inhibitor of PKC, depressed the overflow when given alone and, in addition, attenuated the effect of PMA, 1 mumol/L. The selective alpha 2-adrenoceptor agonist B-HT 933 depressed the overflow; PMA, 1 mumol/L, did not interfere with the effect of B-HT 933, 10 mumol/L. The results provide evidence for the participation of prejunctionally located PKC in the release of noradrenaline. However, PKC does not seem to be involved in the alpha 2-adrenoceptor-agonist-mediated inhibition of noradrenaline release.

• OSTI ID: 5158526
• Journal Information: Journal of Cardiovascular Pharmacology; (United States), Journal Name: Journal of Cardiovascular Pharmacology; (United States) Vol. 17:6; ISSN 0160-2246; ISSN JCPCD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ANIMALS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERS
HORMONES
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SECRETION
SYMPATHOMIMETICS
TIME DEPENDENCE
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES