Interactions between macrophage/Kupffer cells and hepatocytes in surgical sepsis

Thesis/Dissertation · Thu Dec 31 23:00:00 EST 1987

OSTI ID:5156936

West, M A

Experiments were performed to investigate the role of Kupffer cell/macrophage interactions with hepatocytes in modulating liver function during infections using direct in vitro cocultivation of rat macrophages or Kupffer cells with rat hepatocytes. Protein synthesis was assayed as a sensitive indicator of integrated hepatocellular function by measuring {sup 3}H-leucine incorporation into hepatocyte protein. Septic stimuli such as lipoploysaccharide and killed bacteria were added to cocultures of hepatocytes and macrophages or Kupffer cells and the responses compared to hepatocytes alone. Information about the types of proteins synthesized by hepatocytes under various culture conditions was determined using polyacrylamide gel electrophoresis and autoradiography. These experiments showed that septic stimuli alter the amount and type of protein synthesized by hepatocytes and had no direct effect on hepatocytes in the absence of macrophages or Kupffer cells. The mediator(s) appears to be a heat labile, soluble monokine(s) which is distinct from interleukin-1 or tumor necrosis factor. The important role of Kupffer cells/macrophages in mediating alterations in hepatocellular function in sepsis may ultimately improve patient care.

• Research Organization: Minnesota Univ., Minneapolis, MN (USA)
• OSTI ID: 5156936
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BACTERIAL DISEASES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROPHORESIS
FUNCTIONS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
LEUCINE
LIVER CELLS
MACROPHAGES
MAMMALS
MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PHAGOCYTES
PROTEINS
RATS
RODENTS
SOMATIC CELLS
SURGERY
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES