Receptors for epidermal growth factor in the rat uterus

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-117-1-149· OSTI ID:5151863

Mukku, V R; Stancel, G M

Uterine membranes contain high affinity (dissociation constant (Kd) = 0.36 nM), saturable binding sites for (/sup 125/I)iodo-epidermal growth factor (EGF). The binding of (/sup 125/I) iodo-EGF is specific since it is abolished by excess unlabeled EGF but not by excess unlabeled insulin, fibroblast growth factor, or multiplication-stimulating activity. Incubation of (/sup 125/I) iodo-EGF with uterine membranes, followed by chemical cross-linking with disuccinimidyl suberate and detergent extraction reveals a major species of specifically bound EGF (mol wt = 170,000) and a minor species (mol wt = 150,000) visualized by autoradiography of sodium dodecyl sulfate gels after electrophoresis of the extracts. In detergent-solubilized preparations EGF also stimulates the phosphorylation of major (mol wt = 170,000) and minor (mol wt = 150,000) species of identical molecular weight. The increased phosphorylation produced by incubation of membrane extracts with EGF occurs largely at tyrosine residues, as indicated by phosphoamino acid analysis. These results indicate that the rat uterus contains high affinity EGF binding sites with the properties expected of EGF receptors.

• Research Organization: Univ. of Texas Medical School, Houston
• OSTI ID: 5151863
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 1; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
EPIDERMIS
EPITHELIUM
FEMALE GENITALS
FIBROBLASTS
HORMONES
HYDROXY ACIDS
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORYLATION
PITUITARY HORMONES
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESIDUES
RODENTS
SKIN
SOMATIC CELLS
STH
TISSUES
TRACER TECHNIQUES
TYROSINE
UTERUS
VERTEBRATES