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Biosynthesis of trypanosoma brucei variant surface glycoproteins: N-glycosylation and addition of a phosphatidylinositol membrane anchor

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:5150699
The variant surface glycoproteins (VSGs) of Trypanosoma brucei are synthesized with a hydrophobic COOH-terminal peptide that is cleaved and replaced by a glycophospholipid, which anchors VSG to the surface membrane. The kinetics of VSG processing were studied by metabolic labeling with (/sup 35/S)methionine and (/sup 3/H)myristic acid. The COOH-terminal oligosaccharide-containing structure remaining after phospholipase removal of dimyristyl glycerol from membrane-form VSG could be detected serologically within 1 min of polypeptide synthesis in two T. brucei variants studied. Addition of the oligosaccharide-containing structure was resistant to tunicamycin. VSGs synthesized in the presence of tunicamycin displayed lower apparent molecular weights, consistent with the complete inhibition of N-glycosylation at one (variant 117), two (variant 221), or at least three (variant 118) internal asparagine sites. In dual-labeling studies, cycloheximide caused rapid inhibition of both (/sup 35/S)methionine and (/sup 3/H)myristic acid incorporation.
Research Organization:
Rockefeller Univ., New York, NY
OSTI ID:
5150699
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 261:1; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
ASPARAGINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
DATA
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
DRUGS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
GLUCOPROTEINS
HYDROLASES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LIPASE
LIPOTROPIC FACTORS
METHIONINE
MOLECULAR WEIGHT
MONOCARBOXYLIC ACIDS
NUCLEI
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TETRADECANOIC ACID
TRACER TECHNIQUES
TRYPANOSOMES