(4B-3H) NADH-H2O exchange reaction of the mitochondrial NADH dehydrogenase
The purified mitochondrial NADH dehydrogenase enzyme has been shown to catalyze a rapid (4B-/sup 3/H) NADH-H/sub 2/O exchange reaction. When the enzyme is subjected to a single freeze-thaw cycle there is a complete loss of NADH dehydrogenation without a measurable decrease in the (4B-/sup 3/H) NADH-H/sub 2/O exchange. Complete loss of the (4B-/sup 3/H) NADH-H/sub 2/O exchange follows brief exposure to ultraviolet photoirradiation. The differential sensitivity of the water exchange reaction and the dehydrogenase activity suggests a direct involvement of the enzymes flavin cofactor in the catalysis of the (4B-/sup 3/H) NADH-H/sub 2/O exchange. Arylazido-beta-alanyl NAD+ (A3'-0-(3-(N-4-azido-2-nitrophenyl)amino) propionyl)NAD+) is shown to be a potent photodependent inhibitor of the (4B-3H) NADH-H/sub 2/O exchange activity following photoirradiation with visible light. This is consistent with the observed photodependent inhibition of the dehydrogenase activity by this photoprobe.
- Research Organization:
- Univ. of Hawaii, Honolulu
- OSTI ID:
- 5148934
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZIDES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CATTLE
COENZYMES
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FREEZING
HYDROGEN COMPOUNDS
IN VITRO
MAMMALS
NAD
NITROGEN COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RUMINANTS
THAWING
ULTRAVIOLET RADIATION
VERTEBRATES
VISIBLE RADIATION
WATER