Immune response of mice and sheep to bluetongue virus inactivated by gamma irradiation

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Prog. Clin. Biol. Res.; (United States)

OSTI ID:5148747

Campbell, C H; Barber, T L; Knudsen, R C; Swaney, L M

Gamma irradiation is being tested as a means of inactivating bluetongue virus (BTV) for use in vaccines. Exposure of BTV 17 to various levels of irradiation revealed that a dose of approximately 0.6 megarad was required to reduce the virus titer by one log10, or 90%. To test the immunogenicity of irradiated BTV, mouse brain passaged virus and concentrated cell culture passaged virus were inactivated by 6 megarads of gamma irradiation, and vaccines were prepared by emulsifying the virus preparations in equal volumes of a modified incomplete Freund's adjuvant. These vaccines stimulated the production of neutralizing antibodies in mice and sheep, a cell mediated immune response in mice, and a protective immune response in sheep. The results suggest that gamma irradiation would be an effective means of inactivating BTV for the preparation of vaccines.

• Research Organization: Dept. of Agriculture, Greenport, NY
• OSTI ID: 5148747
• Journal Information: Prog. Clin. Biol. Res.; (United States), Journal Name: Prog. Clin. Biol. Res.; (United States); ISSN PCBRD
• Country of Publication: United States
• Language: English

Related Subjects

560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CELL CULTURES
CENTRAL NERVOUS SYSTEM
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
MAMMALS
MICE
MICROORGANISMS
NERVOUS SYSTEM
ORGANS
PARASITES
RADIATION EFFECTS
RADIATIONS
RODENTS
RUMINANTS
SHEEP
VACCINES
VERTEBRATES
VIRUSES