Response of spermatozoa to hyposmotic stress reflects cryopreservation success

Conference · Wed Jan 01 04:00:00 EST 1992

OSTI ID:5148230

Watson, P F; Curry, M R ^[1]; Noiles, E E; Critser, J K ^[2]; Mazur, P ^[3]; Hammerstedt, R H ^[4]

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1. Royal Veterinary College, Dept. of Veterinary Basic Sciences, London (UK)
2. Methodist Hospital of Indiana, Inc., Indianapolis, IN (United States). Center for Reproduction
3. Oak Ridge National Lab., TN (United States)
4. Pennsylvania State Univ., University Park, PA (United States). Dept. of Cellular and Molecular B

Spermatozoa of several species were washed and then subjected to dilution in hyposmotic Tyrode's based solutions. The cells were stained with fluorescent viability stains, carboxyfluorescein diacetate and propidium iodide, and proportions with intact plasma membranes determined by flow cytometry or fluorescence microscopy. Fowl spermatozoa remained almost 100% intact until very low osmolality, and then ruptured. Human spermatozoa showed a similar response with only a small decrease in intact cells before the precipitous decline at low osmolality. Bull spermatozoa were more readily disrupted at higher osmolality, some 40% being damaged before the sudden decline at low osmolality. Ram and boar spermatozoa were progressively disrupted even at mild hyposmotic stress, showing approximately 50% of cells ruptured at 150 mOsm.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5148230
• Report Number(s): CONF-920885-1; ON: DE92014464
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIRDS
CRYOGENICS
FAILURES
FOWL
GAMETES
GERM CELLS
MAMMALS
MAN
MEMBRANES
PRESERVATION
PRIMATES
RUPTURES
SPERMATOZOA
VERTEBRATES
VIABILITY