Effects of antioxidants on glutathione-S-transferase activities in hepatocyte culture

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5146436

Chen, L H ^[1]

---

1. Univ. of Kentucky, Lexington (United States)

Hepatocyte cultures from control rats and rats injected with 3-methylcholanthrene(3-MC) were used to study the effects of antioxidants on the activity of glutathione-S-transferases (GSH-S-T). This group of enzymes catalyzes conjugation of xenobiotics or their metabolites with reduced glutathione and plays an important role in detoxification of xenobiotics. In Experiment 1, treatment of hepatocyte cultures from both control and 3-MC-injected rats with 25 {mu}M or 50 {mu}M butylated hydroxyanisole (BHA) for 24 hours or 48 hours significantly increased GSH-S-T activity with I-chloro-2,4-dinitrobenzene (CDNB) as the substrate. In Experiment 2, treatment of hepatocytes from both control and 3-MC-treated rats with 25 {mu}M ethoxyquin or vitamin E, but not vitamin A or ascorbic acid, significantly increased GSH-S-T activity when CDNB, 1,2-dichloro-4-nitrobenzene or p-nitrobenzyl chloride was used as the substrate, respectively. The results suggested that BHA, ethoxyquin and vitamin E may have detoxification effects against 3-MC-induced carcinogenesis.

• OSTI ID: 5146436
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIOXIDANTS
AROMATICS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CELL CULTURES
CONDENSED AROMATICS
DRUGS
ENZYME ACTIVITY
ENZYMES
GLUTATHIONE
HYDROCARBONS
LIVER CELLS
MAMMALS
METABOLISM
METABOLITES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYCYCLIC AROMATIC HYDROCARBONS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES