Hapten-specific B lymphocytes, enrichment, cloning, receptor analysis, and tolerance induction

Conference · Sat Jan 01 04:00:00 EST 1977 · Cold Spring Harbor Symp. Quant. Biol.; (United States)

OSTI ID:5145902

Nossal, G J.V.; Pike, B L; Stocker, J W; Layton, J E; Goding, J W

In this paper, we review experiments verifying clonal selection through the use of hapten-fractionated B lymphocytes of such uniformity that up to one cell in three can be induced to form an anti-hapten antibody-forming clone in vitro; the use of such cells in studies on antigen-induced receptor aggregation and modulation using immunogenic or tolerogenic antigen concentrations; application to the tolerance problem of the cloning methods devised for the study of these cells; studies of the IgM- and IgD-like receptors of hapten-specific cells; and the use of an anti-delta allotype serum to study the distribution and function of IgD-like molecules on the B-lymphocyte surface.

• Research Organization: Walter and Eliza Hall Inst. of Medical Research, Melbourne
• OSTI ID: 5145902
• Conference Information: Journal Name: Cold Spring Harbor Symp. Quant. Biol.; (United States) Journal Volume: 41
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
GLOBULINS
IMMUNE REACTIONS
IMMUNOGLOBULINS
LEUKOCYTES
LYMPHOCYTES
MOLECULES
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
TOLERANCE