Enhanced recognition of spin trapped radicals in complex mixtures: Deuterated nitronyl adducts provide a gas chromatographic/mass spectrometric marker

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Analytical Letters; (USA)

DOI:https://doi.org/10.1080/00032718908051384· OSTI ID:5143956

Krygsman, P H; Janzen, E G; Towner, R A; Haire, D L ^[1]

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1. Univ. of Guelph, Ontario (Canada)

The use of deuterated analogues of {alpha}-phenyl-N-tert-butyl nitrone (PBN) are reported for GC/MS analysis of spin trapping products. PBN-d{sub 9} and PBN-d{sub 14}, deuterated in the tert-butyl group (the latter also deuterated in the phenyl ring), result in a more diagnostic fragment ion, C{sub 4}D{sub 9}{sup +} (m/z = 66) which improves the recognition of PBN adducts in mixtures. This feature has helped identify a new 1,3-radical addition product of PBN-d{sub 9} formed during azobis(isobutyronitrile) thermolysis (addition of 2-cyano-2-propyloxyl followed by 2-cyano-2-propyl radicals). The {sup 13}C-trichloromethyl radical adduct of PBN-d{sub 14} produced during {sup 13}C-carbon tetrachloride incubation with rat liver microsomes was identified by GC/MS in a complicated biological extract mixture. 25 refs., 3 figs., 1 tab.

• OSTI ID: 5143956
• Journal Information: Analytical Letters; (USA), Journal Name: Analytical Letters; (USA) Vol. 22:4; ISSN ANALB; ISSN 0003-2719
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400202* -- Isotope Effects
Isotope Exchange
& Isotope Separation
AMINES
CARBON 13
CARBON ISOTOPES
CHROMATOGRAPHY
DATA
DEUTERIUM
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
GAS CHROMATOGRAPHY
HYDROGEN ISOTOPES
INFORMATION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MASS SPECTROSCOPY
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRAPPING