Optimization of batch alcoholic fermentation of glucose syrup substrate. [Box-Wilson central composite design]

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260230810· OSTI ID:5135443

Chen, S L

The quantitative effects of substrate concentration, yeast concentration, and nutrient supplementation on ethanol content, fermentation time, and ethanol productivity were investigated in a Box-Wilson central composite design experiment, consisting of five levels of each variable. The highest ethanol productivity of about 21 g EtOH/liter hr was obtained at low substrate concentration (i.e., 12/degree/Brix), low alcohol content (i.e., 6% by weight), high yeast concentration (i.e., 4.4%), and high supplementation of yeast extract (i.e., 6% by weight), high yeast concentration (i.e., 4.4%), and high supplementation of yeast extract (i.e., 2.8%). Productivity of this magnitude is substantially higher than that of the traditional batch fermentation or fed-batch fermentation. It is comparable to the results of continuous fermentation but lower than those of vacuum fermentation. Optimal conditions for maximal ethanol productivity can be established by a multiple regression analysis technique and by plotting the contours of constant response to conform to the constraints of individual operations. 12 refs.

• Research Organization: Univers Foods Corp, Milwaukee, Wis, USA
• OSTI ID: 5135443
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:8; ISSN BIBIA
• Country of Publication: United States
• Language: English

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ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
OPTIMIZATION
ORGANIC COMPOUNDS
PLANTS
PRODUCTION
SACCHARIDES
SUBSTRATES
YEASTS