Assessment of the microscreen phage-induction assay for screening hazardous wastes (1989)
The Microscreen phage-induction assay, which quantitatively measures the induction of prophage Lambda in Escherichia coli WP2s(Lambda), was used to test 14 crude (unfractionated) hazardous industrial-waste samples for genotoxic activity in the presence and absence of metabolic activation. Eleven of the 14 wastes induced prophage, and induction was observed at concentrations as low as 0.4 picograms per ml. Comparisons of the mutagenic activity of these waste samples in Salmonella and their ability to induce prophage Lambda indicate that the phage-induction assay was a more-sensitive indicator of genetic damage for this group of wastes. All but one of the wastes that were mutagenic to Salmonella were detected by the phage-induction assay, and 5 wastes not mutagenic to Salmonella were genetically active in the phage assay. The enhanced ability of the phage-induction assay to detect genotoxic activity may be related to the constituents comprising these waste samples. Partial chemical characterizations of the wastes showed high concentrations of carcinogenic metals, solvents, and chlorinated compounds, most of which are detected poorly by the Salmonella assay.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 5129818
- Report Number(s):
- PB-90-116179/XAB; EPA-600/D-89/137
- Resource Relation:
- Other Information: Pub. in Symposium on Waste Testing and Quality Assurance, Vol. 2, 1-18(1989). See also PB--88-113378
- Country of Publication:
- United States
- Language:
- English
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Use of the Microscreen phage-induction assay to assess the genotoxicity of 14 hazardous industrial wastes
Use of the microscreen phage-induction assay to assess the genotoxicity of 14 hazardous industrial wastes
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32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
BACTERIOPHAGES
HAZARDOUS MATERIALS
ORGANIC WASTES
METALS
MUTAGEN SCREENING
DOSE-RESPONSE RELATIONSHIPS
ESCHERICHIA COLI
METABOLIC ACTIVATION
ORGANIC COMPOUNDS
SALMONELLA TYPHIMURIUM
TOXICITY
VOLATILE MATTER
BACTERIA
ELEMENTS
MATERIALS
MATTER
MICROORGANISMS
PARASITES
SALMONELLA
SCREENING
VIRUSES
WASTES
550700* - Microbiology
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& Utilization- Industrial & Agricultural Processes- Industrial Waste Management