Variations in the alternative oxidase in chlamydomonas grown in air or high CO sub 2

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.3.958· OSTI ID:5129663

Goyal, A; Tolbert, N E ^[1]

---

1. Michigan State Univ., East Lancing (USA)

Chlamydomonas in the resting phase of growth has an equal capacity of about 15 micromole O{sub 2} uptake per hour per milligram of chlorophyll for both the cytochrome c, CN-sensitive respiration, and for the alternative, salicylhydroxamic acid-sensitive respiration. Alternative respiration capacity was measured as salicylhydroxamic acid inhibited O{sub 2} uptake in the presence of CN, and cytochrome c respiration capacity as CN inhibition of O{sub 2} uptake in the presence of salicylhydroxamic acid. Measured total respiration was considerably less than the combined capacities for respiration. During the log phase of growth on high (2-5%) CO{sub 2}, the alternative respiration capacity decreased about 90% but returned as the culture entered the lag phase. When the alternative oxidase capacity was low, addition of salicylic acid or cyanide induced its reappearance. When cells were grown on low (air-level) CO{sub 2}, which induced a CO{sub 2} concentrating mechanism, the alternative oxidase capacity did not decrease during the growth phase. Attempts to measure in vivo distribution of respiration between the two pathways with either CN or salicylhydroxamic acid alone were inconclusive.

• OSTI ID: 5129663
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:3; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHLAMYDOMONAS
CHLOROPHYCOTA
CYANIDES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GROWTH
MICROORGANISMS
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PLANTS
RESPIRATION
UNICELLULAR ALGAE
VARIATIONS