Variations in the alternative oxidase in chlamydomonas grown in air or high CO sub 2
- Michigan State Univ., East Lancing (USA)
Chlamydomonas in the resting phase of growth has an equal capacity of about 15 micromole O{sub 2} uptake per hour per milligram of chlorophyll for both the cytochrome c, CN-sensitive respiration, and for the alternative, salicylhydroxamic acid-sensitive respiration. Alternative respiration capacity was measured as salicylhydroxamic acid inhibited O{sub 2} uptake in the presence of CN, and cytochrome c respiration capacity as CN inhibition of O{sub 2} uptake in the presence of salicylhydroxamic acid. Measured total respiration was considerably less than the combined capacities for respiration. During the log phase of growth on high (2-5%) CO{sub 2}, the alternative respiration capacity decreased about 90% but returned as the culture entered the lag phase. When the alternative oxidase capacity was low, addition of salicylic acid or cyanide induced its reappearance. When cells were grown on low (air-level) CO{sub 2}, which induced a CO{sub 2} concentrating mechanism, the alternative oxidase capacity did not decrease during the growth phase. Attempts to measure in vivo distribution of respiration between the two pathways with either CN or salicylhydroxamic acid alone were inconclusive.
- OSTI ID:
- 5129663
- Journal Information:
- Plant Physiology; (USA), Vol. 89:3; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON DIOXIDE
BIOLOGICAL EFFECTS
CHLAMYDOMONAS
ENZYME ACTIVITY
CYANIDES
GROWTH
OXIDOREDUCTASES
OXYGEN
RESPIRATION
VARIATIONS
ALGAE
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CHLOROPHYCOTA
ELEMENTS
ENZYMES
MICROORGANISMS
NONMETALS
OXIDES
OXYGEN COMPOUNDS
PLANTS
UNICELLULAR ALGAE
551000* - Physiological Systems
560300 - Chemicals Metabolism & Toxicology