Continuous monitoring, by mass spectrometry, of H/sub 2/ production and recycling in Rhodopseudomonas capsulata

Journal Article · Fri Aug 01 04:00:00 EDT 1980 · J. Bacteriol.; (United States)

OSTI ID:5128520

Jouanneau, Y ^[1]; Kelley, B C; Berlier, Y; Lespinat, P A; Vignais, P M

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1. Centre d'Etudes Nucleaires, Grenoble, France

Hydrogen evolution and consumption by cell and chromatophore suspensions of the photosynthetic bacterium Rhodopseudomonas capsulata were measured with a sensitive and specific mass spectrometric technique which directly monitors dissolved gases. H/sub 2/ production by nitrogenase was inhibited by acetylene and restored by carbon monoxide. An H/sub 2/ evolution activity coupled with HD formation and D/sub 2/ uptake (H-D exchange) was unaffected by C/sub 2/H/sub 2/ and CO. Cultures lacking nitrogenase activity also exhibited H-D exchange activity, which was catalyzed by a membrane-bound hydrogenase present in the chromatophores of R. capsulata. A net hydrogen uptake, mediated by hydrogenase, was observed when electron acceptors such as CO/sub 2/, O/sub 2/, or ferricyanide were present in the medium.

• OSTI ID: 5128520
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 143:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

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560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLENE
ALKYNES
BACTERIA
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CELL CULTURES
CHALCOGENIDES
DEUTERIUM COMPOUNDS
DYNAMIC FUNCTION STUDIES
ENZYME ACTIVITY
ENZYMES
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROGEN PRODUCTION
HYDROGEN TRANSFER
HYDROGENASE
MASS SPECTROMETERS
MEASURING INSTRUMENTS
METABOLISM
MICROORGANISMS
MONITORING
NITROGENASES
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
RHODOPSEUDOMONAS
SPECTROMETERS
TOXICITY