Human circulating monocytes internalize /sup 125/I-insulin in a similar fashion to rat hepatocytes: relevance to receptor regulation in target and nontarget tissues

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · J. Lab. Clin. Med.; (United States)

OSTI ID:5128082

Grunberger, G; Robert, A; Carpentier, J L; Dayer, J M; Roth, A; Stevenson, H C; Orci, L; Gorden, P

Circulating monocytes bind /sup 125/I-insulin in a specific fashion and have been used to analyze the ambient receptor status in humans. When freshly isolated circulating monocytes are incubated with /sup 125/I-insulin and examined by electron microscopic autoradiography, approximately 18% of the labeled material is internalized after 15 minutes at 37 degrees C. By 2 hours at 37 degrees C, approximately one half of the /sup 125/I-insulin is internalized. Internalization occurs also at 15 degrees C but at a slower rate. Furthermore, the monocytes bind and internalize /sup 125/I-insulin in a manner that mirrors that of major target tissues, such as rat hepatocytes. These data suggest that the insulin receptor of the circulating monocyte might be regulated by adsorptive endocytosis in a manner analogous to that of target tissue, such as the liver.

• Research Organization: National Institute of Arthritis, Bethesda, MD
• OSTI ID: 5128082
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 2; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CENTRIFUGATION
CHALCOGENIDES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIVER
MAMMALS
MAN
MATERIALS
MINERALS
MONOCYTES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PRIMATES
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEPARATION PROCESSES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
TRACER TECHNIQUES
VERTEBRATES