On-line radiochemical assay for monoamine oxidase utilizing high-performance liquid chromatography
Journal Article
·
· J. Pharmacol. Methods; (United States)
A fast and sensitive assay for the determination of monoamine oxidase activity was developed. The method is based on the separation and quantitation of /sup 14/C-labeled assay products by high-performance liquid chromatography, which is interfaced directly into a flow-through radioactivity detector. This allows on-line quantitation of the radioactive compounds with picomole sensitivity. The method makes possible the complete separation and detection of the deaminated products of monoamine oxidase A and B substrates benzylamine and 5-hydroxytryptamine, respectively. This assay has been applied to the measurement of monoamine oxidase A and B activities in rat brain.
- Research Organization:
- Orion Pharmaceutical Co. Research Center, Espoo, Finland
- OSTI ID:
- 5127503
- Journal Information:
- J. Pharmacol. Methods; (United States), Journal Name: J. Pharmacol. Methods; (United States) Vol. 4; ISSN JPMED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHROMATOGRAPHY
DRUGS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MITOCHONDRIA
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PYRROLES
RADIOASSAY
RADIOCHEMISTRY
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHROMATOGRAPHY
DRUGS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MITOCHONDRIA
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PYRROLES
RADIOASSAY
RADIOCHEMISTRY
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES
VERTEBRATES