Transposon-containing DNA cloning vector and uses thereof
Patent
·
OSTI ID:512421
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.
- Research Organization:
- Washington University; University of Connecticut
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER60862; FG02-90ER61000
- Assignee:
- Univ. of Connecticut, Storrs, CT (United States); Washington Univ., St. Louis, MO (United States)
- Patent Number(s):
- US 5,645,991/A/
- Application Number:
- PAN: 8-403,582
- OSTI ID:
- 512421
- Country of Publication:
- United States
- Language:
- English
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