The 40-kilodalton To autoantigen associates with nucleotides 21 to 64 of human mitochondrial RNA processing/7-2 RNA in vitro
Journal Article
·
· Molecular and Cellular Biology; (United States)
- Baylor Coll. of Medicine, Houston, TX (United States)
- Scripps Clinic and Research Inst., La Jolla, CA (United States)
A 40-kDa To antigen recognized by sera from some patients with autoimmune diseases is an integral component of both human RNase P and mitochondrial RNA processing (MRP) RNase. Human MRP and RNase P RNAs, synthesized in vitro, readily associate with the To antigen present in the HeLa cell extract. Using this in vitro reconstitution system, the binding site of the To antigen is localized to a 44-nucleotide-long sequence corresponding to nucleotides 21 to 64 of the human MRP RNA. UV cross-linking experiments showed that the To antigen binds directly to MRP RNA and to RNase P (H1) RNA through RNA-protein interactions. Although the MRP RNA and RNase P (H1) RNA show sequence homology in four conserved blocks the To antigen-binding site in MRP RNA does not show any obvious primary sequence homology with H1 RNA. These data suggest that the To antigen binds to a conserved and presumably a common secondary or tertiary structure in human MRP and RNase P RNAs.
- OSTI ID:
- 5118385
- Journal Information:
- Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:10; ISSN MCEBD; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
BLOOD SERUM
CELL CONSTITUENTS
CHEMICAL REACTIONS
CROSS-LINKING
DISEASES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
ETIOLOGY
HELA CELLS
HYDROLASES
IMMUNE SYSTEM DISEASES
MITOCHONDRIA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PATIENTS
PHOSPHODIESTERASES
POLYMERIZATION
PROTEINS
RADIATIONS
RNA
RNA-ASE
ULTRAVIOLET RADIATION
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
BLOOD SERUM
CELL CONSTITUENTS
CHEMICAL REACTIONS
CROSS-LINKING
DISEASES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
ETIOLOGY
HELA CELLS
HYDROLASES
IMMUNE SYSTEM DISEASES
MITOCHONDRIA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PATIENTS
PHOSPHODIESTERASES
POLYMERIZATION
PROTEINS
RADIATIONS
RNA
RNA-ASE
ULTRAVIOLET RADIATION