Genomic organization and sequence of the Gus-s/sup a/ allele of the murine. beta. -glucuronidase gene
The Gus-s/sup ..cap alpha../ allele of the mouse ..beta..-glucuronidase gene exhibits a high degree of inducibility by androgens due to its linkage with the Gus-r/sup ..cap alpha../ regulatory locus. The authors isolated Gus-s/sup ..cap alpha../ on a 28-kilobase pair fragment of mouse chromosome 5 and found that it contains 12 exons and 11 intervening sequences spanning 14 kilobase pairs of this genomic segment. The mRNA cap site was identified by ribonuclease protection and primer extension analyses which revealed an unusually short 5' noncoding sequence of 12 nucleotides. Proximal regulatory sequences in the 5'-flanking DNA and the complete sequence of the Gus-s/sup ..cap alpha../ mRNA transcript were also determined. Comparison of the amino acid sequence determined from the Gus-s/sup ..cap alpha../ nucleotide sequence with that of human ..beta..-glucuronidase indicated that the two human mRNA species differ due to alternate splicing of an exon homologous to exon 6 of the mouse gene.
- Research Organization:
- Israel Oceanographic and Limnological Research, Tel Shikmona, Haifa 31080 (IL)
- OSTI ID:
- 5115330
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCURONIDASE
GENES
GENETIC MAPPING
MAN
MESSENGER-RNA
GENETIC VARIABILITY
MICE
AMINO ACID SEQUENCE
CHROMOSOMES
DNA SEQUENCING
GENE RECOMBINATION PROTEINS
GENE REGULATION
NUCLEOTIDES
RNA-ASE
ANIMALS
BIOLOGICAL VARIABILITY
ENZYMES
ESTERASES
GLYCOSYL HYDROLASES
HYDROLASES
MAMMALS
MAPPING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics