Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof

Patent · Tue Feb 23 00:00:00 EST 1988

OSTI ID:5115289

Santerre, R F; Rao, R N

A recombinant DNA cloning vector is described comprising: (a) a eukaryotic promoter, (b) one or two different structural genes and associated control elements that convey resistance to either or both antibiotics hygromycin B and G418 when transformed into a host cell that is sensitive to either or both antibiotics for which resistance is conveyed, the host cell being susceptible to transformation, cell division, and culture, and (c) a prokaryotic replicon, the replicon being functional when the host cell is prokaryotic.

• Assignee: Eli Lilly and Co., Indianapolis, IN
• Patent Number(s): US 4727028
• OSTI ID: 5115289
• Resource Relation: Patent File Date: Filed date 30 Sep 1983
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
RECOMBINANT DNA
TECHNOLOGY ASSESSMENT
ANTIBIOTICS
CELL DIVISION
DNA-CLONING
GENE OPERONS
REPLICONS
SENSITIVITY
ANTI-INFECTIVE AGENTS
CLONING
DNA
DRUGS
ENZYMES
GENES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
TRANSFERASES
550200* - Biochemistry