Dictyostelium discoideum myosin: Isolation and characterization of cDNAs encoding the essential light chain
The authors used an antibody specific for Dictyostelium discoideum myosin to screen a lambdagt11 cDNA expression library to obtain cDNA clones which encode the Dictyostelium essential myosin light chain (EMLC). The amino acid sequence predicted from the sequence of the cDNA clone showed 31.5% identity with the amino acid sequence of the chicken EMLC. Comparisons of the Dictyostelium EMLC, a nonmuscle cell type, with EMLC sequences from similar MLCs of skeletal- and smooth-muscle origin, showed distinct regions of homology. Much of the observed homology was localized to regions corresponding to consensus Ca/sup 2 +/-binding of E-F hand domains. Southern blot analysis suggested that the Dictyostelium genome contains a single gene encoding the EMLC. Examination of the pattern of EMLC mRNA expression showed that a significant increase in EMLC message levels occurred during the first few hours of development, coinciding with increased actin expression and immediately preceding the period of maximal chemotactic activity.
- Research Organization:
- Dept. of Cell Biology and Anatomy, Northwestern Univ. Medical School, 303 E. Chicago, Avenue, Chicago, IL 60611 (US)
- OSTI ID:
- 5115206
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:2
- Country of Publication:
- United States
- Language:
- English
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