Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.2.778· OSTI ID:5113796

Wadsworth, S C; Rosenthal, L S; Kammermeyer, K L; Potter, M B; Nelson, D J

The authors isolated Drosophila melanogaster genomic sequences with nucleotide and amino acid sequence homology to subunits of vertebrate acetylcholine receptor by hybridization with a Torpedo acetylcholine receptor subunit cDNA probe. Five introns are present in the portion of the Drosophila gene encoding the unprocessed protein and are positionally conserved relative to the human acetylcholine receptor alpha-subunit gene. The Drosophila genomic clone hybridized to salivary gland polytene chromosome 3L within region 64B and was termed AChR64B. A 3-kilobasae poly(A)-containing transcript complementary to the AChR64B clone was readily detectable by RNA blot hybridizations during midembryogenesis, during metamorphosis, and in newly enclosed adults. AChR64B transcripts were localized to the cellular regions of the central nervous system during embryonic, larval, pupal, and adult stages of development. During metamorphosis, a temporal relationship between the morphogenesis of the optic lobe and expression of AChR64B transcripts was observed.

• Research Organization: Cell Biology Group, Worcester Foundation for Experimental Biology, 222 Maple Avenue, Shrewsbury, MA 01545 (US)
• OSTI ID: 5113796
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:2; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMINO ACID SEQUENCE
AMMONIUM COMPOUNDS
ANIMALS
ARTHROPODS
AUTONOMIC NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM
DIPTERA
DROSOPHILA
DRUGS
ESTERS
FLIES
FRUIT FLIES
GENE RECOMBINATION PROTEINS
GENES
GENETIC MAPPING
HYBRIDIZATION
INSECTS
INVERTEBRATES
MAPPING
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEOPROTEINS
ONTOGENESIS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PHENOTYPE
PROTEINS
QUATERNARY COMPOUNDS
RECEPTORS
TRANSCRIPTION