Affinity labeling of human glucosephosphate isomerase with N-bromacetylethanolamine phosphate

Journal Article · Mon Oct 24 04:00:00 EDT 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)91426-7· OSTI ID:5113544

Gibson, D R ^[1]; Talent, J M; Gracy, R W; Hartman, F C

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1. North Texas State Univ., Denton

N-Bromoacetylethanolamine phosphate rapidly and irreversibly inactivates glucosephosphate isomerase in a pseudo first-order fashion. Rate-saturation effects are observed with a minimum half-life of 4.5 minutes and a half-maximal rate of inactivation at 0.056 mM. Substrates, as well as competitive inhibitors, protect the isomerase from inactivation. Using /sup 14/C-labeled N-bromoacetylethanolamine phosphate, the incorporation of approximately one equivalent of inactivator per subunit of isomerase is indicated. After acid hydrolysis, the only modification appears to be the formation of carboxymethyl histidine. These studies indicate that the substrate analogue N-bromoacetylethanolamine phosphate is a specific affinity label that can be used to probe the active site of glucosephosphate isomerase.

• OSTI ID: 5113544
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 78:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALDEHYDES
ANIMALS
BIOCHEMISTRY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHEMISTRY
ENZYME INHIBITORS
ENZYMES
GLUCOSE
HEXOSES
INACTIVATION
ISOMERASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PRIMATES
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES