DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster abelson proto-oncogene homolog
Journal Article
·
· Mol. Cell. Biol.; (United States)
The authors report their molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a protein of 1,520 amino acids with strong sequence similarity to the human c-abl proto-oncogene beginning in the type 1b 5' exon and extending through the region essential for tyrosine kinase activity. When the tyrosine kinase homologous region was expressed in Escherichia coli, phosphorylation of proteins on tyrosine residues was observed with an antiphosphotyrosine antibody. These results show that the abl gene is highly conserved through evolution and encodes a functional tyrosine protein kinase required for Drosophila development.
- Research Organization:
- McArdle Lab. for Cancer Research, Univ. of Wisconsin, Madison, WI 53706 (US)
- OSTI ID:
- 5113146
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:2; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ANTIBODIES
ARTHROPODS
CARBOXYLIC ACIDS
DIPTERA
DNA SEQUENCING
DROSOPHILA
ENZYME ACTIVITY
ENZYMES
FLIES
FRUIT FLIES
GENE RECOMBINATION PROTEINS
GENES
GENETIC MAPPING
HYDROXY ACIDS
INSECTS
INVERTEBRATES
MAPPING
MOLECULAR STRUCTURE
MUTATIONS
NUCLEOPROTEINS
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSFERASES
TYROSINE
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ANTIBODIES
ARTHROPODS
CARBOXYLIC ACIDS
DIPTERA
DNA SEQUENCING
DROSOPHILA
ENZYME ACTIVITY
ENZYMES
FLIES
FRUIT FLIES
GENE RECOMBINATION PROTEINS
GENES
GENETIC MAPPING
HYDROXY ACIDS
INSECTS
INVERTEBRATES
MAPPING
MOLECULAR STRUCTURE
MUTATIONS
NUCLEOPROTEINS
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSFERASES
TYROSINE