Tissue-specific enhancer of the human glycoprotein hormone. cap alpha. -subunit gene: Dependence on cyclic AMP-inducible elements
The authors identified and characterized elements which confer tissue specificity and cyclic AMP (cAMP) responsiveness to the human glycoprotein ..cap alpha..-subunit gene. An enhancer containing an 18-base-pair repeat conferred cAMP responsiveness in a non-tissue-specific fashion. DNase I protection assays revealed DNA-binding factors that bound to this element in both placental and nonplacental cells. It also enhanced the ..cap alpha..-subunit promoter in a tissue-specific manner but had a negligible effect on a heterologous promoter. A unique element found upstream of this enhancer had no independent activity but, in combination with the cAMP - responsive enhancer, distinctly increased the tissue-specific activity of both the ..cap alpha..-subunit promoter and a heterologous promoter. A factor that bound to this upstream element was found in placental but not nonplacental cells. The authors conclude that this novel element acts, perhaps through a specific trans-acting factor, in concert with a cAMP-responsive enhancer to confer tissue specificity to the ..cap alpha..-subunit gene.
- Research Organization:
- Regulatory Biology Lab., The Salk Institute, La Jolla, CA 92037 (US)
- OSTI ID:
- 5111523
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 7:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMP
BIOLOGICAL FUNCTIONS
GLUCOPROTEINS
BIOSYNTHESIS
GENES
ANIMAL CELLS
CHEMICAL BONDS
DNA-ASE
GENE AMPLIFICATION
HUMAN POPULATIONS
PLACENTA
SPECIFICITY
TISSUES
BODY
CARBOHYDRATES
ENZYMES
ESTERASES
FETAL MEMBRANES
FUNCTIONS
HYDROLASES
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
POPULATIONS
PROTEINS
SACCHARIDES
SYNTHESIS
550400* - Genetics
550200 - Biochemistry