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Title: Polychlorinated biphenyl (PCB) effects, PCB congener distributions, and cytochrome P-450 regulation in fish

Abstract

PCB studies were conducted in gonadally mature winter flounder (Pseudopleuronectes americanus) collected from spawning grounds with different degrees of PCB contamination. Flounder from Fox Is., RI, Gaspee Pt., RI and New Bedford, MA (US) had mean hepatic PCB concentrations between 1.6-10.6, 3.8-17.7, and 58-333 {mu}g PCB/g dry liver, respectively. PCB congener distributions in these flounder indicated that PCB disposition was not influenced by reproductive condition, that these migratory fish accumulate a significant portion of their PCB body burden during residence in their spawning grounds, and that flounder selectively metabolize congeners with adjacent meta-, para-unsubstituted carbon atoms. The most significant reproductive effect of PCBs in flounder was a 32 to 52% decrease in the mean ovarian size of highly contaminated females from New Bedford. Plasma levels of the sex hormones, estradiol and testosterone, and of the egg yolk precursor, vitellogenin, as well as hepatic estradiol metabolism (measured as estradiol 2-hydroxylase), showed no relationship to hepatic PCB concentration. PCB effects on flounder P4501A indicated that hepatic PCB concentrations as low as 0.9 {mu}g PCB/g were associated with decreased P4501A catalytic efficiency. Additional suppression of flounder P4501A by estrogens was suggested by depressed levels of P4501A messenger RNA, protein and catalytic activity inmore » highly contaminated female fish with high estradiol titers. The mechanism of P4501A suppression by estrogens was studied in killifish (Fundulus heteroclitus) treated wit {beta}-napthoflavone ({beta}-NF), a P4501A inducer, and/or estradiol.« less

Authors:
Publication Date:
Research Org.:
Boston Univ., MA (United States)
OSTI Identifier:
5109278
Alternate Identifier(s):
OSTI ID: 5109278
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph.D.)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHLORINATED AROMATIC HYDROCARBONS; TOXICITY; FISHES; SENSITIVITY; MIXED-FUNCTION OXIDASES; ENZYME ACTIVITY; BODY BURDEN; ESTRADIOL; METABOLISM; REPRODUCTION; TISSUE DISTRIBUTION; WATER POLLUTION; ANIMALS; AQUATIC ORGANISMS; AROMATICS; DISTRIBUTION; ENZYMES; ESTRANES; ESTROGENS; HALOGENATED AROMATIC HYDROCARBONS; HORMONES; HYDROXY COMPOUNDS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXIDOREDUCTASES; OXYGENASES; POLLUTION; PROTEINS; STEROID HORMONES; STEROIDS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology

Citation Formats

Elskus, A.A.. Polychlorinated biphenyl (PCB) effects, PCB congener distributions, and cytochrome P-450 regulation in fish. United States: N. p., 1992. Web.
Elskus, A.A.. Polychlorinated biphenyl (PCB) effects, PCB congener distributions, and cytochrome P-450 regulation in fish. United States.
Elskus, A.A.. Wed . "Polychlorinated biphenyl (PCB) effects, PCB congener distributions, and cytochrome P-450 regulation in fish". United States. doi:.
@article{osti_5109278,
title = {Polychlorinated biphenyl (PCB) effects, PCB congener distributions, and cytochrome P-450 regulation in fish},
author = {Elskus, A.A.},
abstractNote = {PCB studies were conducted in gonadally mature winter flounder (Pseudopleuronectes americanus) collected from spawning grounds with different degrees of PCB contamination. Flounder from Fox Is., RI, Gaspee Pt., RI and New Bedford, MA (US) had mean hepatic PCB concentrations between 1.6-10.6, 3.8-17.7, and 58-333 {mu}g PCB/g dry liver, respectively. PCB congener distributions in these flounder indicated that PCB disposition was not influenced by reproductive condition, that these migratory fish accumulate a significant portion of their PCB body burden during residence in their spawning grounds, and that flounder selectively metabolize congeners with adjacent meta-, para-unsubstituted carbon atoms. The most significant reproductive effect of PCBs in flounder was a 32 to 52% decrease in the mean ovarian size of highly contaminated females from New Bedford. Plasma levels of the sex hormones, estradiol and testosterone, and of the egg yolk precursor, vitellogenin, as well as hepatic estradiol metabolism (measured as estradiol 2-hydroxylase), showed no relationship to hepatic PCB concentration. PCB effects on flounder P4501A indicated that hepatic PCB concentrations as low as 0.9 {mu}g PCB/g were associated with decreased P4501A catalytic efficiency. Additional suppression of flounder P4501A by estrogens was suggested by depressed levels of P4501A messenger RNA, protein and catalytic activity in highly contaminated female fish with high estradiol titers. The mechanism of P4501A suppression by estrogens was studied in killifish (Fundulus heteroclitus) treated wit {beta}-napthoflavone ({beta}-NF), a P4501A inducer, and/or estradiol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}

Miscellaneous:
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