Effect of glutathione on in vitro metabolism of unsaturated aliphatic nitriles to cyanide

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Bulletin of Environmental Contamination and Toxicology; (United States)

DOI:https://doi.org/10.1007/BF01688943· OSTI ID:5108972

Farooqui, M Y.H.; Massa, E ^[1]

---

1. Univ. of Texas Pan American, Edinburg (United States)

Aliphatic nitriles are widely used as important solvents and intermediates in polymer, plastic, synthetic fibers, resins, dyestuffs, pharmaceuticals and vitamin industries. Occupational exposure to these chemicals is principally through inhalation and dermal routes. Human and animal toxicity studies have suggested that the toxicity of aliphatic nitriles is due to their cyanide (CN{sup {minus}}) liberating capacity under biological conditions. Despite the common property of CN{sup {minus}} liberation, their are marked differences among nitriles, in the amounts of CN{sup {minus}} released to cause poisoning, in the duration of exposure and toxicity signs. The extent of metabolism of unsaturated aliphatic nitrile is influenced by the structural properties of the molecule. The purpose of this study is to investigate the effects of functional groups, the type of unsaturated moiety and availability of sulfhydryls especially glutathione (GSH) on the in vitro rat liver postmitochondrial fraction (microsomes + cytosol) is used to provide cytosolic GHS transferase, an essential enzyme of GSH metabolism.

• OSTI ID: 5108972
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (United States), Journal Name: Bulletin of Environmental Contamination and Toxicology; (United States) Vol. 46:3; ISSN 0007-4861; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CYANIDES
DRUGS
GLUTATHIONE
LIVER CELLS
METABOLISM
MICROSOMES
NITRILES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RIBOSOMES
SOMATIC CELLS