Evidence that cells expressing luteinizing hormone-releasing hormone mRNA in the mouse are derived from progenitor cells in the olfactory placode

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.20.8132· OSTI ID:5102415

Wray, S; Grant, P; Gainer, H ^[1]

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1. National Institute of Neurological Disorders and Stroke, Bethesda, MD (USA)

In situ hybridization histochemistry and immunocytochemistry were used to study the prenatal expression of luteinizing hormone-releasing hormone (LHRH) cells in the mouse. Cells expressing LHRH mRNA and peptide product were first detected on embryonic day 11.5 (E11.5) in the olfactory pit. On E12.5, the majority of LHRH cells were located on tracks extending from the olfactory pit to the base of the telencephalon. From E12.5 to E15.5, LHRH cells were detected in a rostral-to-caudal gradient in forebrain areas. Prior to E12.5, cells expressing LHRH mRNA were not detected in forebrain areas known to contain LHRH cells in postnatal animals. Quantitation of cells expressing LHRH mRNA showed that the number of labeled cells on E12.5 (approximately 800) equaled the number of LHRH cells in postnatal animals, but more than 90% of these cells were located in nasal regions. Between E12.5 and E15.5, the location of LHRH cells shifted. The number of LHRH cells in the forebrain increased, while the number of LHRH cells in nasal regions decreased over this same period. These findings establish that cells first found in the olfactory pit and thereafter in forebrain areas express the LHRH gene and correspond to the position of LHRH immunopositive cells found at these developmental times. To further examine the ontogeny of the LHRH system, immunocytochemistry in combination with (3H)thymidine autoradiography was used to determine when LHRH cells left the mitotic cycle. We show that LHRH neurons exhibit a discrete time of birth, suggesting that they arise as a single neuronal population between E10.0 and E11.0. Postnatal LHRH neurons were birth-dated shortly after differentiation of the olfactory placode and before LHRH mRNA was expressed in cells in the olfactory pit.

• OSTI ID: 5102415
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:20; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LH
GENES
NERVE CELLS
BIOLOGICAL LOCALIZATION
OLFACTORY BULBS
CELL DIFFERENTIATION
AUTORADIOGRAPHY
BRAIN
CELL CYCLE
DNA HYBRIDIZATION
DNA REPLICATION
MESSENGER-RNA
MICE
ONTOGENESIS
PREGNANCY
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BODY
CENTRAL NERVOUS SYSTEM
GONADOTROPINS
HETEROCYCLIC COMPOUNDS
HORMONES
HYBRIDIZATION
HYDROGEN COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques